Hotspot

ycf2

LSC · 32-7180 · 7149 bp

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Pi 0.0697

MarkerSeek score 69.50

Primer available yes

Label ycf2

Feature Metadata

label_name: ycf2
feature_type: gene
region: LSC
length_bp: 7149
variable_sites: 498
pi: 0.0697
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2_p1	TAGCTTCGGACGATCCAGAA	CTTGGAACGGAGATCGACAC	50.0	55.0	58.2	58.4	750-760 bp	1.0	81.0
ycf2_p2	TCTTTCGGTCGGGAATAGGT	CTTGGAACGGAGATCGACAC	50.0	55.0	58.1	58.4	578 bp	0.5	55.9
ycf2_p3	CGTCTTTCGGTCGGGAATAG	CTTGGAACGGAGATCGACAC	55.0	55.0	58.2	58.4	580 bp	0.5	55.7
ycf2_p4	CTTTCGGTCGGGAATAGGTC	CTTGGAACGGAGATCGACAC	55.0	55.0	57.8	58.4	577 bp	0.5	55.3
ycf2_p5	GTCTTTCGGTCGGGAATAGG	CTTGGAACGGAGATCGACAC	55.0	55.0	57.8	58.4	579 bp	0.5	55.3