Hotspot

ycf1

SSC · 113628-118499 · 4872 bp

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Pi 0.0563

MarkerSeek score 67.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 4872
variable_sites: 274
pi: 0.0563
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	ACACAATCCAGATCAGGGGA	ACCTTTCCATCCCCATCTGA	50.0	50.0	58.0	58.0	1270 bp	0.5	59.7
ycf1_p2	GAGATCGAAGGCTGATCGTC	ACCTTTCCATCCCCATCTGA	55.0	50.0	58.0	58.0	1224 bp	0.5	59.5
ycf1_p3	TTGGTTGCGAGAAGGTCTTC	ACCTTTCCATCCCCATCTGA	50.0	50.0	58.1	58.0	960 bp	0.5	58.9
ycf1_p4	TACTTCGCCCAAAGTGGAAG	ACCTTTCCATCCCCATCTGA	50.0	50.0	57.8	58.0	1178 bp	0.5	58.5
ycf1_p5	ACACAATCCAGATCAGGGGA	TGAGACCAGAATTCCGAGGA	50.0	50.0	58.0	57.8	1603 bp	0.5	57.9