Hotspot

ndhF

SSC · 129410-131725 · 2316 bp

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Pi 0.0622

MarkerSeek score 72.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2316
variable_sites: 144
pi: 0.0622
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGTGGATGGGAGAGAAAGA	TCCCCCGAAATGCTTTTTGA	50.0	45.0	58.1	58.0	2417-2438 bp	1.0	84.4
ndhF_p2	TCGGGGCTAATATCTTCGGT	TCCCCCGAAATGCTTTTTGA	50.0	45.0	58.0	58.0	2825 bp	0.5	59.9
ndhF_p3	AGACGTGGATGGGAGAGAAA	TCCCCCGAAATGCTTTTTGA	50.0	45.0	58.1	58.0	2419 bp	0.5	59.5
ndhF_p4	ACGTGGATGGGAGAGAAAGA	GAGGTAAGCAGCGATTGTGA	50.0	50.0	58.1	58.0	2990 bp	0.5	59.4
ndhF_p5	AGACGTGGATGGGAGAGAAA	GAGGTAAGCAGCGATTGTGA	50.0	50.0	58.1	58.0	2992 bp	0.5	59.4