Hotspot

trnP-GGG-rpl32

SSC · 127983-128396 · 414 bp

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Pi 0.1

MarkerSeek score 76.40

Primer available yes

Label trnP-GGG-rpl32

Feature Metadata

label_name: trnP-GGG-rpl32
feature_type: igs
region: SSC
length_bp: 414
variable_sites: 36
pi: 0.1
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 76.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-GGG-rpl32_p1	TTCGAACCGAGATGCGTTAG	ACCGAAGATATTAGCCCCGA	50.0	50.0	58.1	58.0	1198-1243 bp	1.0	88.2
trnP-GGG-rpl32_p2	GAGCAACGTGTCTACCTGTT	ACCGAAGATATTAGCCCCGA	50.0	50.0	57.8	58.0	1165-1210 bp	1.0	88.0
trnP-GGG-rpl32_p3	CGAAGCTGTGCGTTAACATG	ACCGAAGATATTAGCCCCGA	50.0	50.0	58.1	58.0	1653-1706 bp	1.0	86.8
trnP-GGG-rpl32_p4	GTAATTGCGCCCAACCAATC	ACCGAAGATATTAGCCCCGA	50.0	50.0	58.1	58.0	1674 bp	0.5	59.3
trnP-GGG-rpl32_p5	GTTAGCATGGATCCATCGGG	ACCGAAGATATTAGCCCCGA	55.0	50.0	58.2	58.0	1558 bp	0.5	58.5