Hotspot

ndhD-ccsA

SSC · 126284-126624 · 341 bp

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Pi 0.151

MarkerSeek score 77.70

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 341
variable_sites: 48
pi: 0.151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	TATGAGCGGTTTCGTGTCAG	CCCTCGCGAAAGAAATAGCT	50.0	50.0	58.0	58.1	1090 bp	0.5	59.5
ndhD-ccsA_p2	TATGAGCGGTTTCGTGTCAG	TCCCTCGCGAAAGAAATAGC	50.0	50.0	58.0	58.1	1091 bp	0.5	59.4
ndhD-ccsA_p3	ATGGGTTTTGTCTCGATCGG	CCCTCGCGAAAGAAATAGCT	50.0	50.0	58.0	58.1	1323 bp	0.5	59.3
ndhD-ccsA_p4	TATGAGCGGTTTCGTGTCAG	GATTGGTTGGGCGCAATTAC	50.0	50.0	58.0	58.1	1356 bp	0.5	59.3
ndhD-ccsA_p5	ATGGGTTTTGTCTCGATCGG	TCCCTCGCGAAAGAAATAGC	50.0	50.0	58.0	58.1	1324 bp	0.5	59.3