Hotspot

rpl20-rps12

LSC · 74104-75024 · 921 bp

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Pi 0.0778

MarkerSeek score 89.70

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 921
variable_sites: 71
pi: 0.0778
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 89.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	TGAGCTCCCTGAAATCCTGA	AACAAAATCCCCTGCCCTTC	50.0	50.0	58.0	58.4	1065-1083 bp	1.0	82.7
rpl20-rps12_p2	TGAGCTCCCTGAAATCCTGA	GAACAAAATCCCCTGCCCTT	50.0	50.0	58.0	58.4	1066-1084 bp	1.0	82.7
rpl20-rps12_p3	AGCCGTACGGAAGAGTTTTG	AACAAAATCCCCTGCCCTTC	50.0	50.0	58.2	58.4	1087-1105 bp	1.0	81.3
rpl20-rps12_p4	AGCCGTACGGAAGAGTTTTG	GAACAAAATCCCCTGCCCTT	50.0	50.0	58.2	58.4	1088-1106 bp	1.0	81.3
rpl20-rps12_p5	TGAGCTCCCTGAAATCCTGA	TAGAACAAAATCCCCTGCCC	50.0	50.0	58.0	57.5	1068-1086 bp	1.0	81.3