Hotspot

ndhC-trnV-UAC

LSC · 58157-58723 · 567 bp

Back to Marattia

Pi 0.0861

MarkerSeek score 80.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 567
variable_sites: 47
pi: 0.0861
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGCGCCCATAGCAATAACAT	AACAAGAGAGCCCAATGCAA	45.0	45.0	58.0	58.0	1410 bp	0.5	59.8
ndhC-trnV-UAC_p2	TGCGCCCATAGCAATAACAT	ACAAGAGAGCCCAATGCAAA	45.0	45.0	58.0	58.0	1409 bp	0.5	59.8
ndhC-trnV-UAC_p3	CGGGCCCAATTGGAAAAATC	AACAAGAGAGCCCAATGCAA	50.0	45.0	58.0	58.0	1178 bp	0.5	59.8
ndhC-trnV-UAC_p4	CGGGCCCAATTGGAAAAATC	ACAAGAGAGCCCAATGCAAA	50.0	45.0	58.0	58.0	1177 bp	0.5	59.8
ndhC-trnV-UAC_p5	GGCGTATCAACCTCGATCTC	ACAAGAGAGCCCAATGCAAA	55.0	45.0	57.9	58.0	1084 bp	0.5	59.6