Hotspot

rps4-trnT-UGU

LSC · 54535-54773 · 239 bp

Back to Marattia

Pi 0.125

MarkerSeek score 78.00

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 239
variable_sites: 28
pi: 0.125
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	GCCTGTTGAACCCTTAGCTT	ATTAATCAAGTCCGCAGCGT	50.0	45.0	58.1	58.0	875-918 bp	1.0	88.6
rps4-trnT-UGU_p2	ATTGTCTAGCTGCGGGAATG	ATTAATCAAGTCCGCAGCGT	50.0	45.0	58.0	58.0	963-1006 bp	1.0	88.3
rps4-trnT-UGU_p3	CTGGCCTGTTGAACCCTTAG	ATTAATCAAGTCCGCAGCGT	55.0	45.0	58.2	58.0	878-921 bp	1.0	87.8
rps4-trnT-UGU_p4	GCCTGTTGAACCCTTAGCTT	CTTCCATCGAGTCTCTGCAC	50.0	55.0	58.1	58.1	1007-1050 bp	1.0	87.2
rps4-trnT-UGU_p5	ATTGTCTAGCTGCGGGAATG	CTTCCATCGAGTCTCTGCAC	50.0	55.0	58.0	58.1	1095-1138 bp	1.0	87.1