Hotspot

trnT-GGU-trnfM-CAU

LSC · 43866-45787 · 1922 bp

Back to Marattia

Pi 0.143

MarkerSeek score 61.50

Primer available yes

Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name: trnT-GGU-trnfM-CAU
feature_type: igs
region: LSC
length_bp: 1922
variable_sites: 71
pi: 0.143
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.26
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnfM-CAU_p1	GAACCGATGACTTACGCCTT	TCAATCGCTTCCACATCAGG	50.0	50.0	58.0	58.0	1286-2696 bp	1.0	88.3
trnT-GGU-trnfM-CAU_p2	GAACCGATGACTTACGCCTT	CTTGAGGTCACGGGTTCAAA	50.0	50.0	58.0	58.0	605-2011 bp	1.0	88.1
trnT-GGU-trnfM-CAU_p3	GAACCGATGACTTACGCCTT	TTCCATCTCGCTCGAAAGTG	50.0	50.0	58.0	58.0	2535 bp	0.5	59.9
trnT-GGU-trnfM-CAU_p4	GAACCGATGACTTACGCCTT	CCGTCTTCTACTAGCAGGGA	50.0	55.0	58.0	57.9	2581 bp	0.5	59.5
trnT-GGU-trnfM-CAU_p5	GAACCGATGACTTACGCCTT	TCCGTCTTCTACTAGCAGGG	50.0	55.0	58.0	57.9	2582 bp	0.5	59.5