Hotspot

psbZ-trnS-UGA

LSC · 39349-39743 · 395 bp

Back to Marattia

Pi 0.0887

MarkerSeek score 77.80

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: LSC
length_bp: 395
variable_sites: 33
pi: 0.0887
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 77.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	CTACTTGACCAACCATCGGG	GGGAGGAGAGAGAGGGATTC	55.0	60.0	58.0	58.0	571-594 bp	1.0	88.1
psbZ-trnS-UGA_p2	CTACTTGACCAACCATCGGG	CGTTTTTGGGATCTTCGTGC	55.0	50.0	58.0	58.0	1070-1106 bp	1.0	87.9
psbZ-trnS-UGA_p3	CTACTTGACCAACCATCGGG	GCGTTTTTGGGATCTTCGTG	55.0	50.0	58.0	58.0	1071-1107 bp	1.0	87.9
psbZ-trnS-UGA_p4	CTACTTGACCAACCATCGGG	ACGAATGGGAGGAGAGAGAG	55.0	55.0	58.0	57.9	577-600 bp	1.0	87.6
psbZ-trnS-UGA_p5	TCTTTTCCTTCGAGAGTGCG	GGGAGGAGAGAGAGGGATTC	50.0	60.0	57.9	58.0	1044-1074 bp	1.0	86.5