Hotspot

psbM-trnD-GUC

LSC · 37500-38156 · 657 bp

Back to Marattia

Pi 0.0842

MarkerSeek score 82.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 657
variable_sites: 55
pi: 0.0842
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	GGGATTAGAATGAACAATGCAGT	GGTGCTCTAACCGATTGAACT	39.1	47.6	57.2	58.0	739-743 bp	1.0	47.4
psbM-trnD-GUC_p2	GGGATTAGAATGAACAATGCAGT	GGATTGACGGGTCTCGAAC	39.1	57.9	57.2	58.0	782-786 bp	1.0	46.9
psbM-trnD-GUC_p3	AGCAGTAGGGATTAGAATGAACA	GGTGCTCTAACCGATTGAACT	39.1	47.6	57.0	58.0	746-750 bp	1.0	46.4
psbM-trnD-GUC_p4	AGAATGAACAATGCAGTAGC	GGTGCTCTAACCGATTGAACT	40.0	47.6	54.0	58.0	733-737 bp	1.0	46.4
psbM-trnD-GUC_p5	AGCAGTAGGGATTAGAATGAACA	GGATTGACGGGTCTCGAAC	39.1	57.9	57.0	58.0	789-793 bp	1.0	46.0