Hotspot

matK-trnK-UUU

LSC · 11265-11927 · 663 bp

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Pi 0.0501
MarkerSeek score 80.20
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
663
variable_sites
32
pi
0.0501
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TCCGAGATATTACATAGTGCA TCGGTCATCGTTCAAATGGG 38.1 50.0 53.7 58.3 725-748 bp 1.0 46.1
matK-trnK-UUU_p2 TCCGAGATATTACATAGTGCAA TCGGTCATCGTTCAAATGGG 36.4 50.0 54.5 58.3 725-748 bp 1.0 46.1
matK-trnK-UUU_p3 TCCGAGATATTACATAGTGCA TCTACCTCGGTCATCGTTCA 38.1 50.0 53.7 57.9 731-754 bp 1.0 46.0
matK-trnK-UUU_p4 TCCGAGATATTACATAGTGCAA TCTACCTCGGTCATCGTTCA 36.4 50.0 54.5 57.9 731-754 bp 1.0 46.0
matK-trnK-UUU_p5 TCCGAGATATTACATAGTGCAATCT TCTACCTCGGTCATCGTTCA 36.0 50.0 57.5 57.9 731-754 bp 1.0 46.0