Hotspot

rpl32-trnL-UAG

SSC · 102308-102854 · 547 bp

Back to Marathrum

Pi 0.0203
MarkerSeek score 66.10
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
547
variable_sites
11
pi
0.0203
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GCCAAATCTGTTTCAACTGGG AGCGTGTCTACCGATTTCAC 47.6 50.0 58.0 58.0 645-649 bp 1.0 73.5
rpl32-trnL-UAG_p2 GCCAAATCTGTTTCAACTGGG CAGCGTGTCTACCGATTTCA 47.6 50.0 58.0 58.0 646-650 bp 1.0 73.5
rpl32-trnL-UAG_p3 GCCAAATCTGTTTCAACTGGG TCCTAAGAGCAGCGTGTCTA 47.6 50.0 58.0 57.9 655-659 bp 1.0 72.6
rpl32-trnL-UAG_p4 GCCAAATCTGTTTCAACTGGG GCTCTTGCACTGCTTCCTAA 47.6 50.0 58.0 58.2 669-673 bp 1.0 72.1
rpl32-trnL-UAG_p5 GCCAAATCTGTTTCAACTGGG TTGCACTGCTTCCTAAGAGC 47.6 50.0 58.0 58.2 665-669 bp 1.0 72.1