Hotspot

rps11-rpl36

LSC · 74728-74899 · 172 bp

Back to Marathrum

Pi 0.0204

MarkerSeek score 65.10

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 172
variable_sites: 3
pi: 0.0204
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	GCGTGAACCTGTTTTGACTG	ATACGTAGACGGGGTCGAAT	50.0	50.0	57.9	58.0	246-271 bp	1.0	83.9
rps11-rpl36_p2	TTTGACTGAGGGTTTTGCCA	ATACGTAGACGGGGTCGAAT	45.0	50.0	57.8	58.0	234-259 bp	1.0	83.5
rps11-rpl36_p3	GCGTGAACCTGTTTTGACTG	CGTTTGATACGTAGACGGGG	50.0	55.0	57.9	58.2	252-277 bp	1.0	82.7
rps11-rpl36_p4	TGCGTGAACCTGTTTTGACT	ATACGTAGACGGGGTCGAAT	45.0	50.0	58.3	58.0	247-272 bp	1.0	82.7
rps11-rpl36_p5	TTTGACTGAGGGTTTTGCCA	CGTTTGATACGTAGACGGGG	45.0	55.0	57.8	58.2	240-265 bp	1.0	82.4