Hotspot

petD-rpoA

LSC · 73026-73210 · 185 bp

Back to Marathrum

Pi 0.0541

MarkerSeek score 67.20

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 185
variable_sites: 10
pi: 0.0541
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTACTGTCGTAGCCCTTTG	CCGTATTCATGCCTGTTCGA	55.0	50.0	58.0	58.1	760-761 bp	1.0	81.0
petD-rpoA_p2	GTACTGTCGTAGCCCTTTGG	CCGTATTCATGCCTGTTCGA	55.0	50.0	58.0	58.1	759-760 bp	1.0	81.0
petD-rpoA_p3	GTCGTAGCCCTTTGGTTAGG	CCGTATTCATGCCTGTTCGA	55.0	50.0	58.0	58.1	754-755 bp	1.0	80.9
petD-rpoA_p4	GTACTGTCGTAGCCCTTTGG	ATAGACGCCGTATTCATGCC	55.0	50.0	58.0	57.9	766-767 bp	1.0	80.9
petD-rpoA_p5	TAATGGTTTCAGTACCCGCG	CCGTATTCATGCCTGTTCGA	50.0	50.0	58.0	58.1	867-868 bp	1.0	80.8