Hotspot

accD-psaI

LSC · 55523-56247 · 725 bp

Back to Marathrum

Pi 0.0138

MarkerSeek score 65.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 10
pi: 0.0138
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GGTACCAGACGGTTCACAAG	GTCATTGCAATTGCCGGAAA	55.0	45.0	58.2	57.9	917-921 bp	1.0	78.8
accD-psaI_p2	ACCAGACGGTTCACAAGAAG	GTCATTGCAATTGCCGGAAA	50.0	45.0	57.8	57.9	914-918 bp	1.0	78.5
accD-psaI_p3	GGTACCAGACGGTTCACAAG	GAAGTCATTGCAATTGCCGG	55.0	50.0	58.2	58.4	920-924 bp	1.0	76.4
accD-psaI_p4	ACCAGACGGTTCACAAGAAG	GAAGTCATTGCAATTGCCGG	50.0	50.0	57.8	58.4	917-921 bp	1.0	76.2
accD-psaI_p5	AGGTACCAGACGGTTCACAA	GTCATTGCAATTGCCGGAAA	50.0	45.0	58.6	57.9	918-922 bp	1.0	75.8