Hotspot

trnQ-UUG-trnK-UUU

LSC · 50388-51031 · 644 bp

Back to Marathrum

Pi 0.0187
MarkerSeek score 70.00
Primer available yes
Label trnQ-UUG-trnK-UUU

Feature Metadata

label_name
trnQ-UUG-trnK-UUU
feature_type
igs
region
LSC
length_bp
644
variable_sites
12
pi
0.0187
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnQ-UUG-trnK-UUU_p1 CAAAATGAATGGGACGTGGC AAAGCCGAGTACTCTACCGT 50.0 50.0 58.0 58.2 759-766 bp 1.0 80.2
trnQ-UUG-trnK-UUU_p2 CAAAATGAATGGGACGTGGC AAGCCGAGTACTCTACCGTT 50.0 50.0 58.0 58.2 758-765 bp 1.0 80.2
trnQ-UUG-trnK-UUU_p3 CAAAATGAATGGGACGTGGC TACCGTTGAGTTAGCAACCC 50.0 50.0 58.0 57.5 745-752 bp 1.0 77.9
trnQ-UUG-trnK-UUU_p4 CAAAATGAATGGGACGTGGC AGTCGCACTTAAAAGCCGAG 50.0 50.0 58.0 58.6 770-777 bp 1.0 76.9
trnQ-UUG-trnK-UUU_p5 CAAAATGAATGGGACGTGGC TCGCACTTAAAAGCCGAGTA 50.0 45.0 58.0 57.3 768-775 bp 1.0 75.7