Hotspot

psbI-psbK

LSC · 49202-49710 · 509 bp

Back to Marathrum

Pi 0.0246

MarkerSeek score 70.00

Primer available yes

Label psbI-psbK

Feature Metadata

label_name: psbI-psbK
feature_type: igs
region: LSC
length_bp: 509
variable_sites: 12
pi: 0.0246
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 70.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbK_p1	CTCACGACCAGGATTACGTC	GTTTGGCAAGCTGCTGTAAG	55.0	50.0	57.8	57.9	626-644 bp	1.0	82.3
psbI-psbK_p2	CTCACGACCAGGATTACGTC	TTCACAAAATTGCCCGAAGC	55.0	45.0	57.8	57.9	716-734 bp	1.0	81.0
psbI-psbK_p3	CTCACGACCAGGATTACGTC	CACAAAATTGCCCGAAGCTT	55.0	45.0	57.8	57.8	714-732 bp	1.0	81.0
psbI-psbK_p4	CTCACGACCAGGATTACGTC	TGGCAAGCTGCTGTAAGTTT	55.0	45.0	57.8	58.3	623-641 bp	1.0	80.3
psbI-psbK_p5	CTCACGACCAGGATTACGTC	TTGGCAAGCTGCTGTAAGTT	55.0	45.0	57.8	58.3	624-642 bp	1.0	80.3