Hotspot

trnC-GCA-rpoB

LSC · 28711-29678 · 968 bp

Back to Marathrum

Pi 0.0213

MarkerSeek score 61.60

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 968
variable_sites: 20
pi: 0.0213
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAAGGATTTGCAGTCCCCC	CCTTCATTTCTATCCACCAGCA	50.0	45.5	58.4	58.1	1035-1046 bp	1.0	64.0
trnC-GCA-rpoB_p2	AAAAGGATTTGCAGTCCCCC	TCCTTCATTTCTATCCACCAGC	50.0	45.5	58.4	57.8	1036-1047 bp	1.0	63.6
trnC-GCA-rpoB_p3	TTGTTAACATAGGCGGCACC	CCTTCATTTCTATCCACCAGCA	50.0	45.5	58.5	58.1	1072-1083 bp	1.0	62.3
trnC-GCA-rpoB_p4	TTGTTAACATAGGCGGCACC	TCCTTCATTTCTATCCACCAGC	50.0	45.5	58.5	57.8	1073-1084 bp	1.0	62.0
trnC-GCA-rpoB_p5	AAAAGGATTTGCAGTCCCCC	CCTTCATTTCTATCCACCAGC	50.0	47.6	58.4	56.4	1035-1046 bp	1.0	59.9