Hotspot

psbZ-trnS-UGA

LSC · 20908-21283 · 376 bp

Back to Marathrum

Pi 0.0187

MarkerSeek score 57.30

Primer available yes

Label psbZ-trnS-UGA

Feature Metadata

label_name: psbZ-trnS-UGA
feature_type: igs
region: LSC
length_bp: 376
variable_sites: 7
pi: 0.0187
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnS-UGA_p1	AGCAATTAACGCAAAAACAGC	CAAGACCGGAGCTATCAACC	38.1	55.0	56.9	58.1	454 bp	1.0	63.9
psbZ-trnS-UGA_p2	GCAATTAACGCAAAAACAGCT	CAAGACCGGAGCTATCAACC	38.1	55.0	56.9	58.1	453 bp	1.0	63.9
psbZ-trnS-UGA_p3	AGCAATTAACGCAAAAACAGC	CTATCAACCACTCGGCCATC	38.1	55.0	56.9	58.1	443 bp	1.0	63.4
psbZ-trnS-UGA_p4	GCAATTAACGCAAAAACAGCT	CTATCAACCACTCGGCCATC	38.1	55.0	56.9	58.1	442 bp	1.0	63.4
psbZ-trnS-UGA_p5	TGTTACTTGACCATCCATCAGG	CAAGACCGGAGCTATCAACC	45.5	55.0	57.8	58.1	521 bp	1.0	63.0