Hotspot

ycf3-psaA

LSC · 14229-15002 · 774 bp

Back to Marathrum

Pi 0.0116

MarkerSeek score 65.40

Primer available yes

Label ycf3-psaA

Feature Metadata

label_name: ycf3-psaA
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 9
pi: 0.0116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3-psaA_p1	CAGAAGCCTGGTTCGATCAA	GCCCATTCCTCGAAAGAAGT	50.0	50.0	57.9	57.9	967-978 bp	1.0	79.0
ycf3-psaA_p2	AGCGCTTACTCCGGAAAATT	GCCCATTCCTCGAAAGAAGT	45.0	50.0	57.9	57.9	921-932 bp	1.0	79.0
ycf3-psaA_p3	CAATAGCGCTTACTCCGGAA	GCCCATTCCTCGAAAGAAGT	50.0	50.0	57.8	57.9	925-936 bp	1.0	78.1
ycf3-psaA_p4	AGAAGCCTGGTTCGATCAAG	GCCCATTCCTCGAAAGAAGT	50.0	50.0	57.6	57.9	966-977 bp	1.0	76.7
ycf3-psaA_p5	TGGAAACAAGCAATAGCGCT	GCCCATTCCTCGAAAGAAGT	45.0	50.0	58.5	57.9	935-946 bp	1.0	76.1