Hotspot

trnV-UAC-ndhC

LSC · 7688-7905 · 218 bp

Back to Marathrum

Pi 0.0291

MarkerSeek score 68.50

Primer available yes

Label trnV-UAC-ndhC

Feature Metadata

label_name: trnV-UAC-ndhC
feature_type: igs
region: LSC
length_bp: 218
variable_sites: 6
pi: 0.0291
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-UAC-ndhC_p1	GGTTCGAATCCGTATAGCCC	TCGGCGATAAAATTCCAGAA	55.0	40.0	57.9	55.2	328-340 bp	1.0	61.0
trnV-UAC-ndhC_p2	GGTTCGAATCCGTATAGCCC	TCGGCGATAAAATTCCAGAAA	55.0	38.1	57.9	56.0	328-340 bp	1.0	59.4
trnV-UAC-ndhC_p3	CGGTTCGAATCCGTATAGCC	TCGGCGATAAAATTCCAGAA	55.0	40.0	58.3	55.2	329-341 bp	1.0	59.1
trnV-UAC-ndhC_p4	GGTTCGAATCCGTATAGCCC	ATCGGCGATAAAATTCCAGA	55.0	40.0	57.9	54.9	329-341 bp	1.0	59.1
trnV-UAC-ndhC_p5	GGTTCGAATCCGTATAGCCC	ATCGGCGATAAAATTCCAGAA	55.0	38.1	57.9	55.7	329-341 bp	1.0	57.6