Hotspot

ycf1

SSC · 131425-136941 · 5517 bp

Back to Maranthes

Pi 0.0035

MarkerSeek score 40.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5517
variable_sites: 29
pi: 0.0035
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TAGAAGGTTTTGGATGAACC	CCCTTTGGTTCTCCTCGAAA	40.0	50.0	52.8	57.4	186 bp	1.0	41.7
ycf1_p2	AGAAATAGAAGGTTTTGGATGAACC	CCCTTTGGTTCTCCTCGAAA	36.0	50.0	57.5	57.4	191 bp	1.0	41.7
ycf1_p3	TAGAAGGTTTTGGATGAACC	ACAGTTTGGGGAATGGAAGC	40.0	50.0	52.8	58.4	213 bp	1.0	41.5
ycf1_p4	AGAAATAGAAGGTTTTGGATGAACC	ACAGTTTGGGGAATGGAAGC	36.0	50.0	57.5	58.4	218 bp	1.0	41.5
ycf1_p5	ATAGAAGGTTTTGGATGAACC	ACAGTTTGGGGAATGGAAGC	38.1	50.0	53.5	58.4	214 bp	1.0	41.5