Hotspot

psbB

LSC · 77101-78627 · 1527 bp

Back to Maranthes

Pi 0.00131

MarkerSeek score 36.80

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: LSC
length_bp: 1527
variable_sites: 3
pi: 0.00131
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	TCCCCGAGATAATCCACTGA	AGAGGAGAGGTGAAAAGTAAAAGA	50.0	37.5	57.2	57.3	1663 bp	1.0	40.1
psbB_p2	TCCCCGAGATAATCCACTGAA	AGAGGAGAGGTGAAAAGTAAAAGA	47.6	37.5	58.0	57.3	1663 bp	1.0	40.1
psbB_p3	TTCCCCGAGATAATCCACTGA	AGAGGAGAGGTGAAAAGTAAAAGA	47.6	37.5	58.0	57.3	1664 bp	1.0	40.1
psbB_p4	TCCTTTCCCCGAGATAATCCA	AGAGGAGAGGTGAAAAGTAAAAGA	47.6	37.5	57.9	57.3	1668 bp	1.0	40.1
psbB_p5	TCCCCGAGATAATCCACTGA	AGGAGAGGTGAAAAGTAAAAGATCA	50.0	36.0	57.2	57.9	1661 bp	1.0	40.1