Hotspot

atpF

LSC · 11797-13155 · 1359 bp

Back to Maranthes

Pi 0.00515

MarkerSeek score 51.60

Primer available yes

Label atpF

Feature Metadata

label_name: atpF
feature_type: gene
region: LSC
length_bp: 1359
variable_sites: 10
pi: 0.00515
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF_p1	TGCCTACAATTACACTAGAAGGA	ATCTATAAAATAAGAGGAGATCCA	39.1	29.2	56.7	52.2	1387-1407 bp	1.0	41.8
atpF_p2	TGCCTACAATTACACTAGAAGGA	TCTATAAAATAAGAGGAGATCCAT	39.1	29.2	56.7	52.2	1386-1406 bp	1.0	41.8
atpF_p3	TGCCTACAATTACACTAGAAGGA	ATCTATAAAATAAGAGGAGATCCAT	39.1	28.0	56.7	52.9	1387-1407 bp	1.0	41.8
atpF_p4	TGCCTACAATTACACTAGAAGG	ATCTATAAAATAAGAGGAGATCCA	40.9	29.2	55.3	52.2	1387-1407 bp	1.0	41.8
atpF_p5	TGCCTACAATTACACTAGAAGG	TCTATAAAATAAGAGGAGATCCAT	40.9	29.2	55.3	52.2	1386-1406 bp	1.0	41.8