Hotspot

ndhF-rpl32

SSC · 118662-119750 · 1089 bp

Back to Maranthes

Pi 0.00562
MarkerSeek score 52.10
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1089
variable_sites
9
pi
0.00562
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGCAAGTGTAACGAAGGGT TTTTTCCGATGTCAAGCCGA 50.0 45.0 58.1 58.1 1341-1350 bp 1.0 80.0
ndhF-rpl32_p2 CGATGCAATAGGTCGTGTGA TTTTTCCGATGTCAAGCCGA 50.0 45.0 58.1 58.1 1805-1814 bp 1.0 79.9
ndhF-rpl32_p3 TCGATGCAATAGGTCGTGTG TTTTTCCGATGTCAAGCCGA 50.0 45.0 58.1 58.1 1806-1815 bp 1.0 79.9
ndhF-rpl32_p4 ATGGGTCAATCAAGTGTCCG TTTTTCCGATGTCAAGCCGA 50.0 45.0 57.9 58.1 1563-1572 bp 1.0 79.8
ndhF-rpl32_p5 CGATGCAATAGGTCGTGTGA AAAAAGCTTTCAACGCGGTC 50.0 45.0 58.1 58.2 1702-1711 bp 1.0 79.6