Hotspot

ndhC-trnV-UAC

LSC · 53397-54542 · 1146 bp

Back to Maranthes

Pi 0.00583

MarkerSeek score 62.70

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1146
variable_sites: 10
pi: 0.00583
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCGGAAATTCGAAATGCCA	GCATGTTGGGTCTTTGAAGC	45.0	50.0	58.2	57.9	1330-1361 bp	1.0	78.8
ndhC-trnV-UAC_p2	TCCGGAAATTCGAAATGCCA	AGAAGGTCTACGGTTCGAGT	45.0	50.0	58.2	57.8	1266-1297 bp	1.0	77.9
ndhC-trnV-UAC_p3	CTCCGGAAATTCGAAATGCC	GCATGTTGGGTCTTTGAAGC	50.0	50.0	57.5	57.9	1331-1362 bp	1.0	76.4
ndhC-trnV-UAC_p4	TCCGGAAATTCGAAATGCCA	TGTTGGGTCTTTGAAGCAGT	45.0	45.0	58.2	57.6	1327-1358 bp	1.0	75.9
ndhC-trnV-UAC_p5	CTCCGGAAATTCGAAATGCC	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.5	57.8	1267-1298 bp	1.0	75.4