Hotspot

petN-psbM

LSC · 30032-31147 · 1116 bp

Back to Maranthes

Pi 0.00122

MarkerSeek score 61.20

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1116
variable_sites: 2
pi: 0.00122
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	GCGCTGTTCATTCTTGTTCC	50.0	50.0	58.2	58.0	1250-1271 bp	1.0	80.0
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	GCGCTGTTCATTCTTGTTCC	50.0	50.0	57.6	58.0	1189-1210 bp	1.0	78.5
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ATTGCTACTGCGCTGTTCAT	50.0	45.0	58.2	58.3	1259-1280 bp	1.0	78.2
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TATTGCTACTGCGCTGTTCA	50.0	45.0	58.2	57.6	1260-1281 bp	1.0	77.2
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	ACTGCGCTGTTCATTCTTGT	50.0	45.0	58.2	58.4	1253-1274 bp	1.0	77.0