Hotspot

atpI-rps2

LSC · 15784-16124 · 341 bp

Back to Maranthes

Pi 0.00978

MarkerSeek score 64.50

Primer available yes

Label atpI-rps2

Feature Metadata

label_name: atpI-rps2
feature_type: igs
region: LSC
length_bp: 341
variable_sites: 5
pi: 0.00978
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpI-rps2_p1	TGAGCATGGACTTGAAACCC	ACGCTATTGCTTCAATCCGA	50.0	45.0	58.1	57.7	439-485 bp	1.0	81.7
atpI-rps2_p2	TGAGCATGGACTTGAAACCC	TGCAATTTGTGAGGGTCGTT	50.0	45.0	58.1	58.3	396-442 bp	1.0	81.7
atpI-rps2_p3	TGAGCATGGACTTGAAACCC	TTGCAATTTGTGAGGGTCGT	50.0	45.0	58.1	58.3	397-443 bp	1.0	81.7
atpI-rps2_p4	TGAGCATGGACTTGAAACCC	GCAATTTGTGAGGGTCGTTC	50.0	50.0	58.1	57.7	395-441 bp	1.0	81.6
atpI-rps2_p5	GAAATGCTGGCCTACTTCCA	ACGCTATTGCTTCAATCCGA	50.0	45.0	57.9	57.7	404-450 bp	1.0	81.4