Hotspot

trnQ-UUG-psbK

LSC · 5933-6349 · 417 bp

Back to Maranthes

Pi 0.00333

MarkerSeek score 60.60

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 417
variable_sites: 2
pi: 0.00333
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 60.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	ATAGGTATACGCTGGGACGG	TAGGCTTCGGGCAATTTTGT	55.0	45.0	58.5	58.1	573-589 bp	1.0	78.2
trnQ-UUG-psbK_p2	ATAGGTATACGCTGGGACGG	GCTTCGGGCAATTTTGTGAA	55.0	45.0	58.5	57.9	570-586 bp	1.0	77.7
trnQ-UUG-psbK_p3	ATAGGTATACGCTGGGACGG	GTAGGCTTCGGGCAATTTTG	55.0	50.0	58.5	57.7	574-590 bp	1.0	76.6
trnQ-UUG-psbK_p4	GACCAAAACCCGTTGCCTTA	TAGGCTTCGGGCAATTTTGT	50.0	45.0	58.7	58.1	528-544 bp	1.0	76.6
trnQ-UUG-psbK_p5	GACCAAAACCCGTTGCCTTA	GCTTCGGGCAATTTTGTGAA	50.0	45.0	58.7	57.9	525-541 bp	1.0	76.1