Hotspot

clpP

LSC · 73501-75599 · 2099 bp

Back to Manilkara

Pi 0.0055

MarkerSeek score 65.20

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2099
variable_sites: 11
pi: 0.0055
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	TCCAATCGCAGATTTCGTGA	AATCCATTGTTACGTTTCCA	45.0	35.0	57.6	53.1	2103-2198 bp	1.0	44.1
clpP_p2	GGGCTGGTTTAGATCGATCC	AATCCATTGTTACGTTTCCA	55.0	35.0	57.8	53.1	2219-2282 bp	1.0	43.9
clpP_p3	GGGCTGGTTTAGATCGATCC	TCCATTGTTACGTTTCCATATCA	55.0	34.8	57.8	56.0	2217-2280 bp	1.0	43.9
clpP_p4	GGGCTGGTTTAGATCGATCC	AAATCCATTGTTACGTTTCCA	55.0	33.3	57.8	54.0	2220-2283 bp	1.0	43.9
clpP_p5	GGGCTGGTTTAGATCGATCC	ATCCATTGTTACGTTTCCAT	55.0	35.0	57.8	52.8	2218-2281 bp	1.0	43.9