Hotspot

trnL-CAA-ycf15

IRa · 148972-149341 · 370 bp

Back to Manilkara

Pi 0.0162

MarkerSeek score 63.30

Primer available yes

Label trnL-CAA-ycf15

Feature Metadata

label_name: trnL-CAA-ycf15
feature_type: igs
region: IRa
length_bp: 370
variable_sites: 6
pi: 0.0162
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-ycf15_p1	AAATGGTAGACACGCGAGAC	AGGAGAAGGAAGGCTGGAAT	50.0	50.0	58.0	58.0	481 bp	1.0	80.8
trnL-CAA-ycf15_p2	TGAAATGGTAGACACGCGAG	AGGAGAAGGAAGGCTGGAAT	50.0	50.0	58.0	58.0	483 bp	1.0	80.7
trnL-CAA-ycf15_p3	AAATGGTAGACACGCGAGAC	GGAGAAGGAAGGCTGGAATG	50.0	55.0	58.0	58.0	480 bp	1.0	80.5
trnL-CAA-ycf15_p4	TGAAATGGTAGACACGCGAG	GGAGAAGGAAGGCTGGAATG	50.0	55.0	58.0	58.0	482 bp	1.0	80.5
trnL-CAA-ycf15_p5	GCCTTGGTGGTGAAATGGTA	AGGAGAAGGAAGGCTGGAAT	50.0	50.0	58.1	58.0	493 bp	1.0	80.1