Hotspot

ndhF-trnL-UAG

SSC · 116357-118420 · 2064 bp

Back to Manilkara

Pi 0.00996
MarkerSeek score 60.00
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
2064
variable_sites
18
pi
0.00996
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 AAAAGAAGAAGCCCCACTCC TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.7 57.9 1974-2189 bp 1.0 81.3
ndhF-trnL-UAG_p2 CTGGAAGTGGAACAAACGGT TCCTAAGAGCAGCGTGTCTA 50.0 50.0 58.3 57.9 1940-2155 bp 1.0 81.1
ndhF-trnL-UAG_p3 AAACGGTATGATCCACGCAT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.7 57.9 1927-2142 bp 1.0 81.1
ndhF-trnL-UAG_p4 AAGAAGAAGCCCCACTCCTA TCCTAAGAGCAGCGTGTCTA 50.0 50.0 57.7 57.9 1972-2187 bp 1.0 80.9
ndhF-trnL-UAG_p5 AAAAGAAGAAGCCCCACTCC AGCAGCGTGTCTACCAATTT 50.0 45.0 57.7 57.8 1967-2182 bp 1.0 80.9