Hotspot

accD-psaI

LSC · 61666-62556 · 891 bp

Back to Manilkara

Pi 0.00392

MarkerSeek score 63.20

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 891
variable_sites: 3
pi: 0.00392
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	45.0	45.0	57.4	58.0	882-1005 bp	1.0	78.9
accD-psaI_p2	CAGTTCCACGCTTTCTTTCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	57.3	58.0	883-1006 bp	1.0	78.5
accD-psaI_p3	AGTTCCACGCTTTCTTTCCT	AAGGCACAAAAATAGCGGGA	45.0	45.0	57.4	58.1	849-972 bp	1.0	78.5
accD-psaI_p4	AGTTCCACGCTTTCTTTCCT	AGGCACAAAAATAGCGGGAA	45.0	45.0	57.4	58.1	848-971 bp	1.0	78.5
accD-psaI_p5	AGTTCCACGCTTTCTTTCCT	AAGAAGCCATTGCAATTGCC	45.0	45.0	57.4	57.9	888-1011 bp	1.0	78.3