Hotspot

trnM-CAU-atpE

LSC · 55273-55482 · 210 bp

Back to Manilkara

Pi 0.0147

MarkerSeek score 63.70

Primer available yes

Label trnM-CAU-atpE

Feature Metadata

label_name: trnM-CAU-atpE
feature_type: igs
region: LSC
length_bp: 210
variable_sites: 3
pi: 0.0147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-atpE_p1	GCGATAGGTCTCGTTTGGTT	CTCTCAGACGAGCTAGGACA	50.0	55.0	58.0	58.0	366-372 bp	1.0	81.7
trnM-CAU-atpE_p2	GCGATAGGTCTCGTTTGGTT	GGACACGAGTCGAAGCTATC	50.0	55.0	58.0	57.9	351-357 bp	1.0	81.1
trnM-CAU-atpE_p3	CGATGCGATAGGTCTCGTTT	CTCTCAGACGAGCTAGGACA	50.0	55.0	57.9	58.0	370-376 bp	1.0	80.7
trnM-CAU-atpE_p4	CGATGCGATAGGTCTCGTTT	GGACACGAGTCGAAGCTATC	50.0	55.0	57.9	57.9	355-361 bp	1.0	80.2
trnM-CAU-atpE_p5	GCGATAGGTCTCGTTTGGTT	AGAAAGCTGAAGGCAAGAGG	50.0	50.0	58.0	57.8	408-414 bp	1.0	80.1