Hotspot

petN-psbM

LSC · 30086-31317 · 1232 bp

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Pi 0.0117

MarkerSeek score 68.80

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 1232
variable_sites: 13
pi: 0.0117
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 68.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTTAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	58.2	57.6	1276-1394 bp	1.0	72.3
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.6	57.6	1215-1333 bp	1.0	70.8
petN-psbM_p3	TAGTTAGTCTCGCTTGGGCT	TGCTACTGCACTGTTCATTC	50.0	45.0	58.2	56.1	1276-1394 bp	1.0	68.1
petN-psbM_p4	ATGGGGAAGAAGTGGACTCT	TGCTACTGCACTGTTCATTC	50.0	45.0	57.6	56.1	1215-1333 bp	1.0	66.6
petN-psbM_p5	ATAGTTAGTCTCGCTTGGGC	TGCTACTGCACTGTTCATTCT	50.0	42.9	57.1	57.6	1277-1395 bp	1.0	66.4