Hotspot

trnC-GCA-petN

LSC · 29249-29995 · 747 bp

Back to Manilkara

Pi 0.0127

MarkerSeek score 62.40

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 747
variable_sites: 9
pi: 0.0127
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGGGACTGCAAATCCTTTT	AGCCCAAGCGAGACTAACTA	50.0	50.0	58.4	58.2	805-821 bp	1.0	80.4
trnC-GCA-petN_p2	AGTTCAAATCTGGGTGTCGC	AGCCCAAGCGAGACTAACTA	50.0	50.0	58.5	58.2	780-796 bp	1.0	79.5
trnC-GCA-petN_p3	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	866-882 bp	1.0	78.5
trnC-GCA-petN_p4	TATCCTTTTGGCGACATGGC	AGCCCAAGCGAGACTAACTA	50.0	50.0	58.6	58.2	838-854 bp	1.0	78.2
trnC-GCA-petN_p5	AGTTCAAATCTGGGTGTCGC	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.5	57.6	841-857 bp	1.0	77.6