Hotspot

atpH-atpI

LSC · 14530-15672 · 1143 bp

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Pi 0.00545

MarkerSeek score 65.40

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1143
variable_sites: 6
pi: 0.00545
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 65.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.0	58.5	1197-1231 bp	1.0	78.7
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	AGGTGAATCTATGGAGGGCC	45.0	55.0	58.0	58.6	1169-1203 bp	1.0	78.4
atpH-atpI_p3	TACCTTGACCAACTCCAGGT	TTTTTGCAACTTTAGCCGCG	50.0	45.0	57.9	58.5	1253-1287 bp	1.0	78.0
atpH-atpI_p4	TACCTTGACCAACTCCAGGT	AGGTGAATCTATGGAGGGCC	50.0	55.0	57.9	58.6	1225-1259 bp	1.0	77.7
atpH-atpI_p5	AATAACGGAAGCGGCAGAAA	TTTTTGCAACTTTAGCCGCG	45.0	45.0	58.2	58.5	1198-1232 bp	1.0	77.5