Hotspot

trnS-GCU-trnS-CGA

LSC · 9080-9804 · 725 bp

Back to Manilkara

Pi 0.0111

MarkerSeek score 59.90

Primer available yes

Label trnS-GCU-trnS-CGA

Feature Metadata

label_name: trnS-GCU-trnS-CGA
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 8
pi: 0.0111
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 59.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-trnS-CGA_p1	ATTAGCAATCCGCCGCTTTA	AGGAATTAAACCGAAAGAACCCT	45.0	39.1	58.0	58.0	841-845 bp	1.0	57.0
trnS-GCU-trnS-CGA_p2	ATTAGCAATCCGCCGCTTTA	AGGGGGTTAATAGAACGAATCAC	45.0	43.5	58.0	58.0	809-813 bp	1.0	56.8
trnS-GCU-trnS-CGA_p3	ATTAGCAATCCGCCGCTTTA	GGGGGTTAATAGAACGAATCACA	45.0	43.5	58.0	58.2	808-812 bp	1.0	55.3
trnS-GCU-trnS-CGA_p4	GCTTTAGTCCACTCAGCCAT	AGGAATTAAACCGAAAGAACCCT	50.0	39.1	57.6	58.0	827-831 bp	1.0	54.0
trnS-GCU-trnS-CGA_p5	ATTAGCAATCCGCCGCTTTA	AGGGGGTTAATAGAACGAATCA	45.0	40.9	58.0	56.6	809-813 bp	1.0	53.9