Hotspot

accD

LSC · 60304-62031 · 1728 bp

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Pi 0.00309

MarkerSeek score 53.30

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1728
variable_sites: 8
pi: 0.00309
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	TCTTCCGAGAAGAAATGAAA	AAAAATACCTGCTCGCCAGA	35.0	45.0	52.1	57.5	1888 bp	0.666667	26.7
accD_p2	TCTTCCGAGAAGAAATGAAA	AAAATACCTGCTCGCCAGAA	35.0	45.0	52.1	57.5	1887 bp	0.666667	26.7
accD_p3	TCTTCCGAGAAGAAATGAAA	AAATACCTGCTCGCCAGAAA	35.0	45.0	52.1	57.5	1886 bp	0.666667	26.7
accD_p4	TCTTCCGAGAAGAAATGAAA	AATACCTGCTCGCCAGAAAA	35.0	45.0	52.1	57.5	1885 bp	0.666667	26.7
accD_p5	TCTTCCGAGAAGAAATGAAA	ATACCTGCTCGCCAGAAAAA	35.0	45.0	52.1	57.5	1884 bp	0.666667	26.7