Hotspot

ndhG-ndhI

SSC · 125079-125517 · 439 bp

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Pi 0.00152
MarkerSeek score 49.40
Primer available yes
Label ndhG-ndhI

Feature Metadata

label_name
ndhG-ndhI
feature_type
igs
region
SSC
length_bp
439
variable_sites
1
pi
0.00152
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhG-ndhI_p1 TCCCAACGAAAAGGCAGAAT GTCGTTTACCAATGTCAGTAGT 45.0 40.9 57.7 56.1 599-631 bp 1.0 51.0
ndhG-ndhI_p2 TCCCAACGAAAAGGCAGAAT GTCGTTTACCAATGTCAGTAGTTG 45.0 41.7 57.7 58.1 599-631 bp 1.0 49.1
ndhG-ndhI_p3 TCCCAACGAAAAGGCAGAAT TCGTTTACCAATGTCAGTAGTTGA 45.0 37.5 57.7 58.2 598-630 bp 1.0 48.6
ndhG-ndhI_p4 TCCCAACGAAAAGGCAGAAT CGTTTACCAATGTCAGTAGTTGA 45.0 39.1 57.7 56.8 597-629 bp 1.0 48.5
ndhG-ndhI_p5 TCCCAACGAAAAGGCAGAAT TCGTTTACCAATGTCAGTAGTTG 45.0 39.1 57.7 56.8 598-630 bp 1.0 48.5