Hotspot

ccsA-ndhD

SSC · 121573-121843 · 271 bp

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Pi 0.0221

MarkerSeek score 49.30

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 271
variable_sites: 9
pi: 0.0221
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 49.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTCCGCAATTGTCGCTTCTA	GTTGACCGGGTTGAAGCTAT	45.0	50.0	57.9	57.9	431-434 bp	1.0	80.6
ccsA-ndhD_p2	TTCCGCAATTGTCGCTTCTA	ATCAGTTGACCGGGTTGAAG	45.0	50.0	57.9	57.8	435-438 bp	1.0	80.0
ccsA-ndhD_p3	CGCAATTGTCGCTTCTATCG	GTTGACCGGGTTGAAGCTAT	50.0	50.0	57.7	57.9	428-431 bp	1.0	79.2
ccsA-ndhD_p4	TCCGCAATTGTCGCTTCTAT	GTTGACCGGGTTGAAGCTAT	45.0	50.0	57.7	57.9	430-433 bp	1.0	78.9
ccsA-ndhD_p5	CGCAATTGTCGCTTCTATCG	ATCAGTTGACCGGGTTGAAG	50.0	50.0	57.7	57.8	432-435 bp	1.0	78.6