Hotspot

trnL-UAG-ccsA

SSC · 120495-120603 · 109 bp

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Pi 0.0245

MarkerSeek score 57.10

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 109
variable_sites: 4
pi: 0.0245
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	ACAATTGACACGACTGAAAAAGA	50.0	34.8	58.0	57.2	238-242 bp	1.0	52.1
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	ACAATTGACACGACTGAAAAAGA	50.0	34.8	58.0	57.2	237-241 bp	1.0	52.1
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	ACAATTGACACGACTGAAAAAGA	50.0	34.8	57.9	57.2	228-232 bp	1.0	51.3
trnL-UAG-ccsA_p4	GGTAGACACGCTGCTCTTAG	ACAATTGACACGACTGAAAAAGA	55.0	34.8	57.8	57.2	230-234 bp	1.0	50.7
trnL-UAG-ccsA_p5	GTAGACACGCTGCTCTTAGG	ACAATTGACACGACTGAAAAAGA	55.0	34.8	57.8	57.2	229-233 bp	1.0	50.7