Hotspot

atpA-atpF

LSC · 12768-12837 · 70 bp

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Pi 0.00952

MarkerSeek score 50.80

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 70
variable_sites: 1
pi: 0.00952
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	GTCGCCTACTTGAAGTACGG	GCATGTTTGGCGCGATAAAA	55.0	45.0	58.1	58.4	206-216 bp	1.0	80.4
atpA-atpF_p2	CCGTCGCCTACTTGAAGTAC	GCATGTTTGGCGCGATAAAA	55.0	45.0	58.1	58.4	208-218 bp	1.0	80.3
atpA-atpF_p3	TACTAATCTCGTCGGCTCGA	GCATGTTTGGCGCGATAAAA	50.0	45.0	57.8	58.4	126-136 bp	1.0	79.8
atpA-atpF_p4	GCTCAATACGTTCGCGGATA	GCATGTTTGGCGCGATAAAA	50.0	45.0	58.2	58.4	150-160 bp	1.0	79.6
atpA-atpF_p5	GTCGCCTACTTGAAGTACGG	TGTTTGACCAACGAGTTGCA	55.0	45.0	58.1	58.5	252-262 bp	1.0	78.3