Hotspot

trnK-UUU-rps16

LSC · 4356-5186 · 831 bp

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Pi 0.00642
MarkerSeek score 53.60
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
831
variable_sites
8
pi
0.00642
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 CCGATGGTATGGACGAATCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1021-1024 bp 1.0 82.5
trnK-UUU-rps16_p2 CGATGGTATGGACGAATCCC AAAAAGGCGCTCAACCTACA 55.0 45.0 57.9 58.0 1020-1023 bp 1.0 82.5
trnK-UUU-rps16_p3 CCGCACTTAAAAGCCGAGTA AAAAAGGCGCTCAACCTACA 50.0 45.0 58.3 58.0 969-972 bp 1.0 81.0
trnK-UUU-rps16_p4 CGATGGTATGGACGAATCCC CCTTGAAAAAGGCGCTCAAC 55.0 50.0 57.9 58.2 1025-1028 bp 1.0 80.8
trnK-UUU-rps16_p5 CGATGGTATGGACGAATCCC CTTGAAAAAGGCGCTCAACC 55.0 50.0 57.9 58.2 1024-1027 bp 1.0 80.8