Hotspot

rbcL

LSC · 58370-59797 · 1428 bp

Back to Mangifera

Pi 0.0063

MarkerSeek score 59.20

Primer available yes

Label rbcL

Feature Metadata

label_name: rbcL
feature_type: gene
region: LSC
length_bp: 1428
variable_sites: 9
pi: 0.0063
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL_p1	CCAGGGAAGGGTTAACTCCT	ATTGTTGGGTTCGGCTCAAT	55.0	45.0	58.3	58.1	1601 bp	1.0	77.2
rbcL_p2	TCCAGGGAAGGGTTAACTCC	ATTGTTGGGTTCGGCTCAAT	55.0	45.0	58.3	58.1	1602 bp	1.0	77.2
rbcL_p3	CCAGGGAAGGGTTAACTCCT	TGGGTTCGGCTCAATCTTTT	55.0	45.0	58.3	57.7	1596 bp	1.0	75.5
rbcL_p4	CCAGGGAAGGGTTAACTCCT	TTGGGTTCGGCTCAATCTTT	55.0	45.0	58.3	57.7	1597 bp	1.0	75.5
rbcL_p5	TCCAGGGAAGGGTTAACTCC	TGGGTTCGGCTCAATCTTTT	55.0	45.0	58.3	57.7	1597 bp	1.0	75.5