Hotspot

petD

LSC · 79964-81200 · 1237 bp

Back to Mangifera

Pi 0.00737

MarkerSeek score 60.00

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1237
variable_sites: 9
pi: 0.00737
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 60.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACGGACGAATAGTTGAAGGG	TTGACTTTGAAGCGCCTTCT	50.0	45.0	57.3	58.0	1334-1350 bp	1.0	75.9
petD_p2	ACGGACGAATAGTTGAAGGG	CTTTGAAGCGCCTTCTACCT	50.0	50.0	57.3	57.9	1330-1346 bp	1.0	75.4
petD_p3	ACGGACGAATAGTTGAAGGG	TGAAGCGCCTTCTACCTAGA	50.0	50.0	57.3	57.9	1327-1343 bp	1.0	75.0
petD_p4	ACGGACGAATAGTTGAAGGG	ACTTTGAAGCGCCTTCTACC	50.0	50.0	57.3	58.2	1331-1347 bp	1.0	74.6
petD_p5	ACGGACGAATAGTTGAAGGG	CACTTTGACTTTGAAGCGCC	50.0	50.0	57.3	58.2	1338-1354 bp	1.0	74.2