Hotspot

infA

LSC · 83297-83543 · 247 bp

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Pi 0

MarkerSeek score 18.80

Primer available yes

Label infA

Feature Metadata

label_name: infA
feature_type: gene
region: LSC
length_bp: 247
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 18.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infA_p1	CGTGCACTTTTACGTGAACC	AGTTTCATTAGCGCCTGAGG	50.0	50.0	58.0	58.0	1370-1371 bp	1.0	80.5
infA_p2	TCTCCTACGGATCAGTCGAC	AGTTTCATTAGCGCCTGAGG	55.0	50.0	58.0	58.0	1133-1134 bp	1.0	80.4
infA_p3	GTCCCTCTTCTTGTACCCCT	AGTTTCATTAGCGCCTGAGG	55.0	50.0	58.0	58.0	1505-1506 bp	1.0	80.2
infA_p4	CCCTCTTCTTGTACCCCTGA	AGTTTCATTAGCGCCTGAGG	55.0	50.0	58.0	58.0	1503-1504 bp	1.0	80.2
infA_p5	TCCCTCTTCTTGTACCCCTG	AGTTTCATTAGCGCCTGAGG	55.0	50.0	58.0	58.0	1504-1505 bp	1.0	80.2