Hotspot

rpl32-trnL-UAG

SSC · 117440-118133 · 694 bp

Back to Mangifera

Pi 0.0113

MarkerSeek score 56.80

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 694
variable_sites: 6
pi: 0.0113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.77
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	TAGCGAAATCCCTTTCCACG	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	634-797 bp	1.0	84.5
rpl32-trnL-UAG_p2	TAGCGAAATCCCTTTCCACG	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	635-798 bp	1.0	84.4
rpl32-trnL-UAG_p3	GGGCAGCGTTCAAAACTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	58.1	58.0	658-821 bp	1.0	84.1
rpl32-trnL-UAG_p4	GGGCAGCGTTCAAAACTTTT	CAGCGTGTCTACCGATTTCA	45.0	50.0	58.1	58.0	659-822 bp	1.0	84.1
rpl32-trnL-UAG_p5	TAGCGAAATCCCTTTCCACG	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	644-807 bp	1.0	83.5