Hotspot

ndhF-rpl32

SSC · 116299-117265 · 967 bp

Back to Mangifera

Pi 0.0207
MarkerSeek score 59.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
967
variable_sites
20
pi
0.0207
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CTGGAAGTGGAACGAAAGGT TTTAATAGAGAAGTACGCTTTTT 50.0 26.1 57.7 52.2 1054-1149 bp 1.0 41.5
ndhF-rpl32_p2 CTGGAAGTGGAACGAAAGGT TTTTAATAGAGAAGTACGCTTTT 50.0 26.1 57.7 52.2 1055-1150 bp 1.0 41.5
ndhF-rpl32_p3 CTGGAAGTGGAACGAAAGGT TTTTTAATAGAGAAGTACGCTTT 50.0 26.1 57.7 52.2 1056-1151 bp 1.0 41.5
ndhF-rpl32_p4 CTGGAAGTGGAACGAAAGGT TTTTAATAGAGAAGTACGCTTTTT 50.0 25.0 57.7 53.0 1055-1150 bp 1.0 41.5
ndhF-rpl32_p5 CTGGAAGTGGAACGAAAGGT TTTTTAATAGAGAAGTACGCTTTT 50.0 25.0 57.7 53.0 1056-1151 bp 1.0 41.5